1. Assess the effects of activation or repression of thousands of different genes on cell growth or other screenable phenotypes using pools of sgRNA constructs that target the promoter or transcript start regions of genes.
2. Identify potential therapeutic targets responsible for controlling growth and differentiation, regulating disease development, or other biological responses.
3. Dual-sgRNA CRISPRa and CRISPRi libraries extend the range of tools available for more efficient functional genetic screening and accelerate the identification of novel targets for therapeutics and biomarker analysis.